Antioxidants production of Aspergillus spp from Universitas Indonesia Culture Collection (uicc)

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AIP Conference Proceedings 
2023
, 020169 (2018); 
https://doi.org/10.1063/1.5064166
2023
, 020169
© 2018 Author(s).
Antioxidants production of 
Aspergillus
 spp.
from Universitas Indonesia Culture Collection
(UICC)
Cite as: AIP Conference Proceedings 
2023
, 020169 (2018); 
https://doi.org/10.1063/1.5064166
Published Online: 23 October 2018
W. Mangunwardoyo, C. Lasmaria and D. Kusmana
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Antioxidants Production of 
Aspergillus
 spp. from
Universitas Indonesia Culture Collection (UICC) 
W. Mangunwardoyo
1, a)
, C. Lasmaria
2
, and D. Kusmana
1 
1
Department of Biology, Faculty of Mathematics and Natural Sciences (FMIPA),
Universitas Indonesia, Depok, 16424 
2
High School Stella Maris Bumi Serpong Damai, Tangerang, Banten 
 
a)
Corresponding author: [e-mail] 
Abstract
. The aim of this research was to produce antioxidants of 12 strains of 
Aspergillus
spp. from the Universitas 
Indonesia Culture Collection (UICC). The fermentation was carried out with MPGY medium for 14 days in room 
temperature (26-29 °C)
under still culture condition. The filtrate was extracted using ethyl acetate. The result revealed the 
antioxidant yield was the lowest 
A. phoenicus
UICC 13 (1.50%) and the highest was 
A
. 
niger
UICC 371 (3.80%). 
Analysis antioxidants using thin layer chromatography (TLC) the BHT as a standard showed the Rf value of 
Aspergillus 
awamori
UICC 9 has the same Rf value as the standard (0.16). An antioxidants activity analysis using DPPH (2,2 
diphenyl-1-picryhydrazyl) confirmed that 
Aspergillus awamori
UICC 9 has Inhibition Concentration (IC
50
) value of 
1359.5 µg/mL.