Modern
B. taurus Samples. Sampling procedures.
We sampled a total
of 520 native cattle individuals from across the Mediterranean
Basin and Eastern Europe, representing 51 breeds representing
17 countries (see Table 2). To avoid exotic crosses with native
cattle (which are, in general, unlikely to affect the maternal
lineages), we only sampled small countryside villages, taking also
great care to exclude related individuals. After local anesthesia,
small (3 mm
3
) skin biopsies were collected and stored in 95%
ethanol before DNA extraction.
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