Table haplotype composition (GenBank accession number) for each species. For each site, basin, stream, altitude and geographical coordinates are shown; also site location in Múrria et al. (2013) is given. Species

Table S1. Haplotype composition (GenBank accession number) for each species. For each site, basin, stream, altitude and geographical coordinates are shown; also site location in Múrria et al. (2013) is given.

Species	GenBank accession number	Basin	Stream	Altitude	Latitude	Longitude	Site (Múrria et al. 2013)
H. solerorum	JQ687905, KF255657-KF255659	Guadalhorce	Caballos	415	36º40'29"N	04º54'27"W	B52
H. siltalai	HM134823	Llobregat	Llobregat	817	42º14'22"N	01º57'15"E	H05
	HM134823, HM134824	Llobregat	Llobregat	760	42º14'11"N	01º53'46"E	H06
	HM134823, HM134824	Ter	Ges	606	42º04'50"N	02º18'33"E	H08
	HM134829	Ter	Sorreigs	550	42º00'11"N	02º11'46"E	H09
	HM134824	Llobregat	Cardener	336	41º52'47"N	01º42'42"E	H11
	HM134823, HM134824	Tordera	Arbúcies	335	41º49'04"N	02º29'49"E	H12
	HM134823, HM134824, HM134827, HM134828	Besós	Vallcàrquera	376	41º43'41"N	02º16'58"E	H13
	HM134823, HM134825, HM134824	Besós	Cànoves	420	41º42'34"N	02º20'55"E	H14
	JQ687902	Jara	Arquillo	990	38º45'10"N	02º21'45"W	T26
	JQ687902	Segura	Mundo	806	38º30'30"N	02º14'54"W	T27
	KF255651, KF255652	Genil	Juntas	860	37º25'53"N	03º39'08"W	B39
	KF255651, KF255654	Genil	Colomera	780	37º24'08"N	03º43'11"W	B40
	HM134823, KF255651, KF255652, KF255653	Guadiana Menor	Gor	1333	37º20'53"N	02º56'55"W	B41
	KF255649, KF255650	Miel	Miel	220	36º06'42"N	05º30'19"W	B53
H. infernalis	JQ687904	Genil	Genil	1060	37º09'05"N	03º24'50"W	B43
	JQ687904	Guadiana Menor	Tejos	1660	37º07'07"N	03º4'56"W	B44
	JQ687903	Genil	Cerezal	1060	36º55'36"N	03º59'11"W	B48
	JQ687904	Genil	Añales	970	36º55'02"N	03º53'32"W	B49
H. fezana	HM134821	Laou	Laou	560	35º18'42"N	05º21'12"W	R56
	HM134821	Laou	Laou	400	35º14'21"N	05º10'32"W	R59
	HM134821, HM134822	Laou	Laou	905	35º06'48"N	05º11'08"W	R61
H. fontinalis	JQ687908	Guadiana Menor	Guardal	1090	37º53'51"N	02º40'31"W	T35
H. cf. spiritoi	KF255655	Foix	Pontons	540	41º25'12"N	01º30'30"E	H16
	KF255655	Foix	Pontons	660	41º25'01"N	01º31'23"E	H17
	KF255655, KF255656, JQ687906	Ebre	Canaletes	720	40º54'19"N	00º21'32"E	H20
H. instabilis	JQ687922	Llobregat	Llobregat	817	42º14'22"N	01º57'15"E	H05
	KF255635, KF255637	Ter	Ges	606	42º04'50"N	02º18'33"E	H08
	JQ687922	Tordera	Arbúcies	335	41º49'04"N	02º29'49"E	H12
	KF255637	Tordera	Arbúcies	335	41º49'04"N	02º29'49"E	H12
	JQ687922	Besós	Cànoves	420	41º42'34"N	02º20'55"E	H14
	KF255634, KF255636	Ebre	Canaletes	720	40º55'26"N	00º21'29"E	H20
	JQ687921	Guadalfeo	Trevélez	1860	37º00'16"N	03º28'38"W	B46
	JQ687921	Genil	Cerezal	1060	36º55'36"N	03º59'11"W	B48
H. cf. spiritoi	JQ687906	Llobregat	Marganell	321	41º38'21"N	01º47'12"E
	JQ687906	Leza/Ebro	Tejada	882	42º12'33"N	02º30'17"W
H. spiritoi	Hspi1, Hspi2 (KY678901, KY678902)	Mandre	Villadoro	660	37º42'43"N	14º17'51"E
	Hspi3 (KY678903)	Argens	Bresque	376	43º34'57"N	05º08'60"E
	Hspi3 (KY678903)	Durance/Rhône	Calavon	500	43º57'48"N	05º36'22"E
	Hspi3 (KY678903)	Durance/Rhône	Calavon	467	43º56'58"N	05º35'51"E
	Hspi3 (KY678903)	Ouvèze/Rhône	Toulourenc	582	44º10'30"N	05º25'60"E
H. ambigua	HapC11 (KY656176)	Deva	Corvera	550	43º14'16"N	04º38'33"W
	HapC19, HapC42 (KY656184,KY656207)	Nansa	Venta Fresnedo	193	43º16'47"N	04º29'31"W
	HapC36 (KY656201)	Sella	Covadonga	137	43º18'53"N	05º04'18"W
	HapC39 (KY656204)	Cares	Duje	855	43º13'36"N	04º45'22"W
H. tenuis	HapC33 (KY656198)	Ain	Hérisson	785	46º36'33"N	05º53'01"E

Table S2. Complete locality information of individuals morphologically described. For each site, basin, stream and/or locality, altitude and geographical coordinates are shown.

Species	Basin	Stream/Locality	Altitude	Latitude	Longitude
H. solerorum	Guadalhorce	Caballos/Tolox	415	36º40'31''N	04º54'27''O
	Guadalhorce	Caballos/Tolox	340	36º40'50''N	04º54'31''O
	Guadalhorce	Horcajos/Tolox	472	36º41'36''N	04º56'36''O
	Guadalhorce	Pereilas/Coín	206	36º39'16''N	04º47'15''O
	Guadalhorce	Corcho/Coín	428	36º36'37''N	04º47'33''O
H. cf. spiritoi	Llobregat	Coaner/Coaner	630	41º50'04''N	01º43'25''E
	Foix	Pontons/Pontons	660	41º25'04''N	01º31'22''E
	Foix	L’Albareda/Fontrubí	488	41º25'21''N	01º35'36''E
	Llobregat	Cornet/Sant Salvador de Guardiola	240	41º41'35''N	01º47'47''E
	Llobregat	Vilanova/Avinyó	470	41º54'30''N	01º58'26''E
	Llobregat	Sant Cugat/Sant Cugat del Racó	310	41º48'06''N	01º48'13''E
	Llobregat	Can Relat/Sentforas	567	41º54'25''N	02º12'35''E
	Llobregat	Gaià/Gaià	456	41º55'17''N	01º55'59''E
	Llobregat	Marganell/Marganell	321	41º38'23''N	01º47'12''E
	Aragón/Ebro	Bailo	701	42º30'35''N	00º48'46''O
	Mijares	Fuen Lozana/Mora de Rubielos	1037	40º15'10''N	00º45'17''O
	Jalón/Ebro	Piedra/Monasterio de Piedra	741	41º11'40''N	01º47'13''O
	Leza/Ebro	Tejada/Jalón de Cameros	882	42º12'36''N	02º30'17''O
	Segre	Alinyà/Alinyà	931	42º10'48''N	01º25'24''E
H. spiritoi	Durance/Rhône	Calavon/Vailsaintes	500	43º57'51''N	05º36'23''E
	Durance/Rhône	Calavon/Simiane la Rotonde	467	43º56'59''N	05º35'51''E
	Ouvèze/Rhône	Toulourenc/Montbrun les Bains	582	44º10'33''N	05º26'00''E