1- labоratоry work separation of plants and animal proteins by their solubility The purpose of the work

1- LABОRATОRY WORK
Separation of plants and animal proteins by their solubility
The purpose of the work: Solubility of plant and animal proteins and their analysis.


Required reagents:

1. Wheat and peas.

2. Milk production.

3. Homogenized muscle tissues

4. 10% saturated ammonium sulphate (NH₄)₂ SO₄ solution.

5. Dry salt of ammonium sulphate.

6. 0,2%, 1% and 10% sodium hydroxide (NaOH).

7. 0,1 n va 3% acetic acid solution.

8. Biuret reactive.

9. Saturated solution of Na Cl .

10. Dry salt of NaSl.

11. 70% solution of ethyl alcohol.

12. Homogenized muscle tissues

13. 10% and solution of saturated ammonium sulphate (NH₄)₂ SO₄.

14. Dry salt of ammonium sulphate.

15. 0,2%, 1% and 10% sodium hydroxide (NaOH).

16. 0,1 n and 3% acetic acid solution.

17. Biuret reactive.

18. Saturated solution of Na Cl .

19. Dry salt of NaSl.

20 70% solution of ethyl alcohol.

The container and tools: Glass roofs, porcelain pallets, filter paper, gauze, technical scales, thermostat, 100 ml flat tube, pipettes, test tubes, water bath. Glass roofs, porcelain pallets, filter paper, gauze, technical scales, thermostat, 100 ml flat tube, pipettes, test tubes, water bath.
Proteins.

Proteins are important polymers in life. They are composed of amino acids and are linked to peptide bonds. Each type of protein is characterized by a sequence of amino acids in the polypeptide bond (primary protein structure). Protein is a high-molecular biological polymer that contains nitrogen and consists of 20 different amino acids. The fact that their proteins are called Greek protos (primary, important) also indicates the importance of these groups' biological importance. Almost all life processes are related to protein substances and their biological function.

Proteins is subdivided into protein and proteid.

Protein is a simple protein

Proteid is a complex protein
They are an integral part of all living organisms, single-celled aquatic plants and bacteria, multicellular animals, and viruses that surround the human body and living organisms.
1. Albumins. Water-soluble proteins are present in the precipitate when heated. They are the most common proteins found in all cells. The solution is precipitated when the ammonium sulphate is dissolved in a saturated solution. Such proteins are extracted from cereals, legumes, milk, meat, eggs, whey and other biomaterials.

2. Glоbulin. It is soluble in 10% salts, always present in the cells and tissues in combination with alumines, insoluble in water, coagulated when heated, dissolved in diluted salt solutions, and immediately precipitated by increased salt concentration.