SylbV01-00-00. pmd

Block 2:
Spice Crops
Unit I:
In-vitro
mass multiplication techniques: 
In-vitro
conservation of spice
crops. direct and indirect organogenesis, micro grafting, hardening
techniques, production of microrhizomes.
Unit II:
In-vitro
breeding: Production of haploids, somaclones and identification
of somaclonal variants, 
in-vitro
techniques to overcome fertilization
barriers, Protoplast culture and fusion, construction, identification and
characterization of somatic hybrids and cybrids, wide hybridization,

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Horticultural Sciences–Plantation, Spices, Medicinal and Aromatic Crops
463
embryo rescue of recalcitrant species, 
in-vitro
mutation for biotic and
abiotic stresses, disease elimination in crops.
Unit III:
Transgenic crops: Recombinant DNA methodology, gene transfer
methods, tools, methods, applications of rDNA technology. Role of
molecular markers in characterization of transgenic crops,
fingerprinting of cultivars, etc., achievements, problems and future
thrusts.
Block 3:
Medicinal and Aromatic Crops
Unit I:
In-vitro
mass multiplication techniques: 
In-vitro
conservation of
medicinal and aromatic crops, direct and indirect organogenesis, micro
grafting, hardening techniques, production of microrhizomes.
Unit II:
In-vitro
breeding: Production of haploids, somaclones and identification
of somaclonal variants, 
in-vitro
techniques to overcome fertilization
barriers, Protoplast culture and fusion, construction, identification and
characterization of somatic hybrids and cybrids, wide hybridization,
embryo rescue of recalcitrant species, 
in-vitro
mutation for biotic and
abiotic stresses, disease elimination in crops.
Unit III:
Transgenic crops: Recombinant DNA methodology, gene transfer
methods, tools, methods, applications of rDNA technology. Role of
molecular markers in characterization of transgenic crops, finger
printing of cultivars, etc., achievements, problems and future thrusts.
Unit IV:
In-vitro
production of secondary metabolites: 
In-vitro
production and
characterization of secondary metabolites, bioreactors.
Crops
Coconut, Rubber, Oil palm, Coffee, Tea, Cocoa, Black pepper, Cardamom, Turmeric,
Ginger, Vanilla, Periwinkle, Rauvolfia, Mint, Cymbopogon grasses, Medicinal coleus,
Ocimum
sp., Aswagandha, Aloe, Safed musli, Stevia