SylbV01-00-00. pmd

I. Course Title
: Phenomics and Genomics for PGR Utilization*
II. Course Code
: PGR 602
III. Credit Hours
: 2(1+1)
IV. Why this course ?
Utilisation of conserved germplasm all over World has been poor and needs emphasis
for increasing the resilience and productivity of agricultural production systems.
Students need to understand all the advanced techniques in phenotyping and
genotyping to be able to exploit the PGR.
V. Aim of the course
To impart theoretical and practical knowledge on recent advances in crop germplasm
evaluation and use. To teach current advances in genomic technologies in use for
breeding, phylogenetic analyses, understanding genetic value, facilitating germplasm
selection in genebanks, and develop practical skills in phenotyping and genotyping.
VI. Theory
Unit I
Advances in phenotyping to overcome limitations in use of germplasm collections;
advanced methodology of germplasm evaluation and predictive methods for
identification of useful germplasm, phenomics facility, quantitative imaging
techniques using remote sensing. Experimental designs, analyses of evaluation
data and database management.
Unit II
Evaluation of crop germplasm for agronomic traits: Evaluation against biotic/ abiotic
stresses; quality attributes and other value addition traits. Management and
utilization of crop germplasm, germplasm registration, Core and minicore collections;
Germplasm enhancement/ pre-breeding and use of wild relatives in crop
improvement, embryo rescue method, pollen physiology and storage, integration of
big data into breeding programs, harmonising agro-biodiversity conservation and
agricultural development, New crops of the future, biofortified crops.
Unit III
Uses and applications of molecular markers in PGR – analysis of genetic diversity,
identification of gaps in collection, molecular cytology, Establishment of core and
mini-core collections using molecular markers, Identification of desirable genes
and alleles, germplasm characterisation, trait mapping, genome sequencing, High
throughput genotyping – GBS, association mapping studies: GWAS, molecular
tagging of QTLs, FIGS.
VII. Practical
• Management and utilization of crop germplasm: Exercise for developing core set;
• Validation using molecular markers;
• Evaluation of crop germplasm for value addition;
• Evaluation of crop germplasm against biotic/ abiotic stresses;

---

144
Restructured and Revised Syllabi of Post-graduate Programmes
Vol. 1
• Evaluation of germplasm for quality traits;
• Biochemical/ Molecular characterisation of germplasm.