Submission of Proposal under Star College Scheme



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Bog'liq
2.-Chemistry-Final-compilation

Usual Strength:
300 mg (300 mg of ferrous gluconate is approximately equivalent to 35 mg of ferrous iron )


Tests:
Ferric Iron - weigh accurately a quantity of the powder prepared for the assay,containing about5.0 g of ferrous gluconate, in a stoppered flask, dissolve as completely as possible without the aid of heat in a mixture of 100 ml of freshly boiled and cooled water and 10 ml of hydrochloric acid, add 3 g of potassium iodide, close the flask and allow to stand in dark for 5 minutes. Titrate the liberated iodine with 0.1 M sodium thiosulphate using starch solution, added towards the end point of the titration, as indicator. Repeat the operation the operation without the substance under examination. The difference between the titrations represents the amount of iodine liberated by the ferric ion. Not more than 11.2 ml of 0.1 M sodium thiosulphate is required.


Assay:
Weigh and powder the 20 tablets. Weigh accurately a quantity of the powder containing about 1.0 g of ferrous gluconate , dissolve in a mixture of 140 ml of water and 60 ml of 1 M sulphuric acid and titrate with 0.1 M cerric ammonium sulphate, using ferroin as indicator, until the red colour disappears.
1 ml of 0.1 M cerric ammonium sulphate, is equivalent to 0.04822g of C12H22FeO14, 2H2O.


Storage:
Store protected from light.


Labelling:
The label states the quantity of the active ingredient both as the amount of ferrous gluconate and in terms of the equivalent amount of ferrous iron in each tablet.


Project: 3
Aim:
Analysis of cyanocobalamin Injection.
Vitamin B12 injection
Cyanocobalamin Injection is sterile solution of cyanocobalamin in water for injections containing sufficient acetic acid or hydrochloric acid to adjust the pH to about 4. It may contain suitable buffering agents.
Cyanocobalamin injection containnot less than 95.0 percent and not more than 110.0 percent of the stated amount of anhydrous cyanocobalamin,C63H88 CoN14O14P.



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