Phylogeny of the genus gossypium and genome origin

TABLE OF CONTENTS 

 

 

 

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ABSTRACT .........................................................................................     iii 

 

ACKNOWLEDGMENTS....................................................................      v 

 

TABLE OF CONTENTS .....................................................................      vi 

 

LIST OF FIGURES..............................................................................      vii 

 

LIST OF TABLES ...............................................................................      viii 

 

INTRODUCTION................................................................................       1 

 

MATERIALS AND METHODS .........................................................       5 

 

        Plant materials ..............................................................................       5 

        Nuclear DNA isolation.................................................................       5 

        Repeated sequence probes............................................................       8 

        Southern blot preparation and hybridization................................       8 

        Data analysis and phylogenetic tree reconstruction .....................       9 

        Inference of genome origin of polyploid cottons .........................      10 

 

RESULTS.............................................................................................      12 

 

        Restriction profiles of nuclear repetitive DNA sequences  ..........      12 

        Inference of genome origin of polyploid species .........................      23 

        Reconstruction of phylogenetic tree.............................................      27 

 

DISCUSSION AND CONCLUSIONS................................................      35 

 

REFERENCES.....................................................................................      42 

 

VITA ....................................................................................................      45  

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