SylbV01-00-00. pmd

I. Course Title
: Biotechnological Approaches in Plantation, Spice,
Medicinal and Aromatic Crops
II. Course Code
: PSM 605
III. Credit Hours
: (3 +0)
IV. Why this course ?
Tools of biotechnology are widely used in crop improvement, crop management,
crop protection and post harvest management of PSMA crops. This course is designed

---

Restructured and Revised Syllabi of Post-graduate Programmes
Vol. 1
462
to impart knowledge on advanced biotechnological tools used in various spheres of
plantation, spices, medicinal and aromatic crops.
V. Aim of the course
The main objective of the course is to impart to the learner, knowledge on advanced
biotechnological tools used in various spheres of plantation, spices, medicinal and
aromatic crops.
The course is organized as follows:
No
Blocks
Units
1
Plantation Crops
I
In-vitro
mass multiplication techniques
II
In-vitro
breeding
III Transgenic crops
2
Spice Crops
I
In-vitro 
mass multiplication techniques
II
In-vitro
breeding
III Transgenic crops
3
Medicinal and Aromatic Crops
I
In-vitro 
mass multiplication techniques
II
In-vitro
breeding
III Transgenic crops
IV
In-vitro
production of secondary metabolites
VI. Theory
Block 1: Plantation Crops
Unit I:
In-vitro
mass multiplication techniques: 
In-vitro
conservation of
plantation crops, direct and indirect organogenesis, micro grafting,
hardening techniques.
Unit II:
In-vitro
breeding: Production of haploids, somaclones and
identification of somaclonal variants, 
in-vitro
techniques to
overcome fertilization barriers, protoplast culture and fusion,
construction, identification and characterization of somatic hybrids
and cybrids, wide hybridization, embryo rescue of recalcitrant
species. 
In-vitro
mutation for biotic and abiotic stresses, disease
elimination in crops.
Unit III:
Transgenic crops: Recombinant DNA methodology, gene transfer
methods, tools, methods, applications of rDNA technology. Role
of molecular markers in characterization of transgenic crops,
fingerprinting of cultivars, etc., achievements, problems and
future thrusts.