5 Thus, the pack characteristic and territorial move- ments of the wolf population at the model site (f\e As- pantai-Shakaman) play an important role in the

Material and Methods of research

Faunistic research was conducted by a conventional 

route method [2, 3–11]. The material phytohelmintholog-

ical research were samples of wheat plants and rhizosphere 

soil collected in 16 districts and 48 farms in Surkhandarya 

and Kashkadarya regions. In each region in order to collect 

the material were chosen three farms. Field studies were 

carried out in the period from 2012 to 2015.

In the route faunal studies all 864 soil and 3438 plant 

samples were collected. To isolate nematodes from soil 

and plant organs we used a modified Infundibular meth-

od of Berman. Soil samples for the presence of cyst form 

nematodes we usually analyzed according to the method 

of Dekker [3, 221–237].

For fixing the nematode 6.4% formalin, and glycerol 

formulations were prepared according to the procedure 

of Seinchorst [4, 57–69].

In determining the species of plant nematodes accesso-

ries were used works of domestic and foreign authors, as well 

as phytonematodes atlas, compiled by the Institute of Parasi-

tology, Republican Academy of Sciences, as well as we used 

morphometric parameters obtained by the standard formula 

de Mann according to Micoletzky modification [5, 16–38].

The degree of dominance of nematodes  in plant 

and soil samples was determined by the percentage of 

specimens of individual species among all detected. At 

the same time the dominant species or eudominants are 

up more than 10% of the number of detected, domi-

nants — 5,1–10%, subdominants — 2,1 5%, less than 

2.1% subrecedent individuals [2, 3–11].