Қўқон дпи. Илмий хабарлар

Цуцон ДПИ. Илмий хабарлар
IDENTIFICATION OF GYPSOGENINBISDESMOSIDE IN 
ALLOCHRUSA GYPSOPHILOIDES
BY 
ULTRA-HIGH PERFORMANCE LIQUID CHROMATOGRAPHY-ELECTRON SPRAY IONIZATION­
MS/MS
Vahobjon U. Khujaev, Rano Z. Mamadalieva
Kokand State Pedagogical Institute,
Аннотация. 
Allochrusa gypsophiloides
(mahalliy nomi Beh, Etmak) - Markaziy Osiyodagi eng taniqli 
endemik saponinli o'simlik. Ultra yuqori mahsuldor suyuq xromatografiya-elektropurkash ionlash-mass- 
spektrometriya (UPLC-ESI-MS) 
A. gypsophiloides
ildizlari birikmalarini tahlil qilishda qo'llandi. UPLC- 
ESI-MS orqali o'simlik metanol ekstrakti triterpen saponin -3-O-b-D-galaktopiranosil- 
(1 ^ 2 ) - [p -D- 
ksilopiranosil- (1^-3)] - р-D-glukuronopiranosil gipsogenin 28-O-p-D-ksilopiranosil-(1^-4) - [p-D- 
glukopiranosil- (1 ^ 3 )] - a-L-rhamnopirano-sil- (1 ^ 2 ) - [p -D-glukopiranosil - (1 ^ 4 ) ] - р-D-fukopiranosil efiri 
aniqlandi va gipsogenin bisdesmozidi sifatida aniqlandi.
Аннотация.Allochrusagypsophiloides (местное название Бех, Этмак) - наиболее известное 
эндемичное сапониноносное растение Центральной Азии. Сверх высокопроизводительная 
жидкостная хроматография-электрораспыли-тельная ионизация-масс-спектрометрия (UPLC-ESI- 
MS) применялась для анализа соединений корней A. gypsophiloides. Посредством UPLC-ESI-MS 
исследований 
растительного 
метанольного 
экстракта тритерпенового 
сапонина 
-3-O-p-D- 
галактопиранозил- ( 1 ^ 2) - [p-D-ксилопиранозил- 
(1 ^ 3)] - p-D-глюкуронопиранозил гипсогенин 
28Ю -р^-ксилопиранозил- (1 ^ 4) - [p-D-глюкопиранозил- (1 ^ 3)] - a-L-рамнопиранозил- ( 1 ^ 2) - [р- 
D-глюкопиранозил - (1 ^ 4 )] - p-D-фукопиранозиловый эфир были обна-ружены и идентифицированы 
как бисдесмозид.
A nnotation. 
Allochrusa gypsophiloides(local
name Beh, Etmak) is the best known endemic saponin- 
bearing plant of Central Asia. Ultra-high performance liquid chromatography-electrospray ionization-mass 
spectrometry (UPLC-ESI-MS) was applied to the analysis of the compounds of the roots of 
A. 
gypsophiloides.
Through UPLC-ESI-MS studies on the plant methanol extract triterpene saponin -3-O-p-D- 
galactopyranosyl-(1 ^2)-[p-D-xylopyranosyl-(1^3)]-p-D-glucuro-nopyranosyl 
gypsogenin 
28-O-p-D- 
xylopyranosyl-(1^4)-[p-D-glucopyranosyl-(1^3)]-a-L-rhamnopyranosyl-(1^2)-[p-D-glucopyranosyl- 
(1^4)]-p-D-fuco-pyranosyl ester were detected and identified as bisdesmoside of gypsogenin.
Kalit so'zlar: 
Allochrusa gypsophiloides,
UPLC-ESI-MS, saponin, gipsogenin bisdesmosidi, triterpen 
glikozidlari.
Ключевые слова: Allochrusa gypsophiloides, UPLC-ESI-MS, сапонин, гипсогенин бисдесмозид, 
тритерпеновые гликозиды.
Key w ords: 
Allochrusa gypsophiloides,
UPLC-ESI-MS, saponin, gypsogenin bisdesmoside,triterpene 
glycosides
Introduction
Allochrusa
is a genus of family Caryophyllaceae comprising about 8 species distributed in Turkey, 
Central Asia, Afghanistan, Caucasus, and Iran. One species of 
Allochrusagypsophiloides
occurs in Central 
Asia. 
A. gypsophiloides
has a relatively limited area, it grows only in the western Tien Shanand Pamir-Alay 
at foothill desert loess steppes, dry slopes of rivers, on gravelly slopes at analtitude of 400-1300 meters 
above sea level. This species has been known as a valuable source of saponin on the territory of the 
republics of Central Asian countries such as Uzbekistan and Kazakhstan, has been exported for a long 
time. Annual planned volumes of collecting dry roots reached 700 - 800 tones [1]. As a result of the 
intensive and unsystematic harvesting of 
A. gypsophiloides
brings it to the list of rare species with a strongly 
decreasing population and recorded in the Red Book of Uzbekistan [2].
Triterpene glycosides are natural low molecular weight compounds that are widespread in plants 
and some sea animals. Among them 
Allochrusa
are a rich source of triterpene glycosides with diverse 
chemical structures and physiological activities.Although, there are some reports about glycosides 
(triterpene saponins) in this plant [3, 4],UPLC-ESI-MS investigations have not yet been conducted on 
A. 
gypsophiloides.
Therefore, the objectives of this study were to study saponins of the methanol extract of 
A. 
gypsophiloides
using UPLC-ESI-MS. Herein, we report the known saponin in the methanol extract from 
A. 
gypsophiloides
roots.
Results and discussion
Liquid chromatography coupled to mass spectrometry (LC-MS) is an excellent technique to analyse 
multi-components in complex herbal matrices. Due to its inherent characteristics of accurate mass 
measurements and high resolution, time-of-flight (TOF) MS is well-suited to this field, especially for 
qualitative applications[5]. Roots from 
A. gypsophiloideshave
been shown to accumulate triterpenoid 
saponins [3, 4]. They belong to the group of glucuronide quillaic- and gypsogenin type triterpenoid 
carboxylic acid 3,28-O-bidesmosidessaponins. Compounds from this group have a glucuronic acid moiety 
at C-3 hydroxyl of the aglycone and are among the highest glycosylated bidesmosides [6]. Negative ion 
ESI-MS of saponins offers high sensitivity and more diagnostic fragment ions compared to positive ion MS.
journal.kspi.uz 
2020/№1
88

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