Mycoplasma and Ureaplasma Dr. R. K. Kalyan Associate Professor Microbiology Dept. Kgmu mycoplasma

Liquid medium

• PPLO agar base without crystal violet ph 7.8+Yeast extract +Horse serum +Sodium deoxyribonucleate +Thallous acetate solution+K2HPO4, Penicilin solution
• + Glucose serum + Phenol red +Methylene blue.
• Biphasic medium: 1. solid phase- Standard solid medium
• 2. Liquid phase- Liquid medium.

MCQ

• Q.1. Which of the following bacteria was named as Eaton agent
• Acholeplasma
• Mycoplasma hominis
• Mycoplasma pneumoniae
• Ureaplasma urealyticum
• Q.2. Dienes method is used to examine colonies of
• Bordetella
• Burkholderia
• Mycoplasma
• Helicobacter

• Q.3.Which of the following bacteria is/are associated with nongonococcal urethritis ?
• Mycoplasma hominis
• Ureaplasma urealyticum
• Chlamydia trachomatis
• All of the above
• Q.4.Which is the causative agent of primary atypical pneumoniae
• Influenza virus
• Streptococcus Pneumoniae
• Haemophilus influenzae
• Mycoplasma pneumoniae

• Q.5. Which of the following can hydrolyse urea
• Mycoplasma
• Acholeplasma
• Ureaplasma
• Escherichia
• Q.6. Which of the following bacteria is/are also named T strain ?
• Mycoplasma pneumoniae
• Mycoplasma hominis
• Ureaplasma urealyticum
• Acholeplasma

• Q.7.Postpartum fever due to Mycoplasma hominis is treated with
• Penicillin G
• A second generation Cephalosporins
• Vancomycin
• Tetracyclines
• Q.8.A distinguishing feature of human mycoplasma species is that they:
• Stain well with Giemsa, but not by Gram stain
• Contain no bacterial peptidoglycan
• Are not immunogenic because they mimic host cell membrane components
• Cannot be cultivated in vitro

• Q.9. which of the following tests can be used to identify Mycoplasma pneumoniae ?
• Haemadsorption test
• Tetrazolium reduction test
• Inhibition of growth by specific antisera
• All of the above
• Q.10. Which of the following bacteria shows fried egg colonies on culture media ?
• Helicobacter
• Mycobacterium tuberculosis
• Bordetella
• Mycoplasma

ANSWERS OF MCQ

• Q.1- C
• Q.2- C
• Q.3- d
• Q.4- d
• Q.5- C
• Q.6- C
• Q.7- d
• Q.8- b
• Q.9- d
• Q.10- d

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• Thanks for attention !