Molecular Phlogeny of Acantholimon Boiss. (Plumbaginaceae) Genus from Turkey Related trn and its regions

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462 
The 3
rd 
International Symposium on EuroAsian Biodiversity 
05-08 July 2017, Minsk - BELARUS 
PP-274 
Molecular Phlogeny of Acantholimon Boiss. (Plumbaginaceae) Genus from Turkey 
Related TRN and ITS Regions
 
Feyza CANDAN
1
, Tuna UYSAL
2
, Musa DOĞAN
3
, Meryem BOZKURT
2,4
1 
Department of Biology, Faculty of Science and Arts, Manisa Celal Bayar University,
Manisa, Turkey
2 
Department of Biology,Faculty of Science, Selçuk University, Konya, Turkey 
3 Department of Biological Sciences, Faculty of Science and Arts, Middle East Technical 
University, Ankara, Turkey 
4
Advanced Technology Research and Application Center, Selçuk University, Konya, Turkey 
[e-mail] 
Aim of the study:
The impact of molecular phylogenetic studies on plants have raised in the 
meantime development of technology in the light of high explanations regarding 
relationships. According to this study, 
Acantholimon
genus has selected to find out its 
molecular phlogeny by using TRN and ITS primers. According to this study, it is aimed to 
reveal molecular phlogeny of 
Acantholimon
genus by using TRN and ITS primers related 62 
taxa of 
Acantholimon
genus, which of 42 are endemic, collected from Turkey by preparing 
dendrograms and making analysis. 
Material and Methods:
62 taxa of 
Acantholimon
genus were gathered from all over the 
Turkey at their flowering times. Specimens were kept as herbarium materials. Flora of Turkey 
was used for their taxonomical description. On the other hand, DNA isolation was made by 
using Qiagen DNeasy Plant Mini Kit. After DNA deriving, bioanalyzer instrument was used to 
measure their A260/280 rate (purity evaluation) and determine the quality of DNA. However, 
agarose jel photographs were taken after PCR reactions.
TRNc-d and ITS 4b-5a primers were selected for this investigation from the literature checks.
TRNc: CGA AAT CGG TAG ACG CTA CG, 40 nmol 
TRNd: GGG GAT AGA GGG ACT TGA AC, 40 nmol 
ITS 4b: TCCTCCGCTTATTGATATGC,20 nmol 
ITS 5a: GGAAGGAGAAGTCGTAACAAGG,20 nmol 
Cycle sequencing and purity studies of PCR materials after cycle sequencing were made. 
After all these gradations, DNA sequence analyses were made in a detailed way. 
Results:
Molecular phlogeny of 
Acantholimon
genus tried to reveal with the primers as TRN 
c-d region of the chloroplast DNA and ITS 4b-5a region of the nucleus DNA. As a result of 
the study, it is determined the relationships between taxa according to genus. 6 dendrograms 
by using TRN, ITS and both TRN-ITS sequences were 
made with ‘Maximum Possibilty 
Analysis’ and ‘Parsimony Analysis’ to reveal relation degrees according to taxa of genus and 
comparisons were made. Network analysis was made by using TRN and ITS data to 
understand geographic distribution of the genus. 
Acknowledgements: 
The authors would like to thank the Scientific and Technical Research 
Council of Turkey (TUBITAK) for projects funding (TBAG-1781 and 212T222). 
Keywords: Acantholimon
, TRN, ITS, molecular phlogeny.