Mining and Development of Novel ssr markers Using Next Generation Sequencing (ngs) Data in Plants

Document Outline

• Introduction 
  • Importance of Microsatellites and Their Use as Genetic Markers 
  • Next-Generation Sequencing (NGS) 
  • SSR Discovery by Transcriptome Sequencing (RNA-Seq) 
• Overview of the Process of SSR Development through Transcriptome de Novo Assembly Using the Illumina Platform 
  • de Novo Assembly 
  • Unigene Functional Annotation 
  • Microsatellites Mining and Identification Tools 
  • DNA Isolation, PCR Amplification, and SSR Validation 
  • Genotyping STRs in Next-Generation Data: Challenges and Solutions 
• Conclusions 
• References