Improved pFastBac™ donor plasmid vectors for higher protein production using the Bac-to-Bac® baculovirus expression vector system



Download 0,94 Mb.
Pdf ko'rish
bet1/17
Sana28.03.2022
Hajmi0,94 Mb.
#514949
  1   2   3   4   5   6   7   8   9   ...   17
Bog'liq
pFast bac maqola



Accepted Manuscript
Title: Improved pFastBac
TM
donor plasmid vectors for higher
protein production using the Bac-to-Bac® baculovirus
expression vector system
Authors: Hui Shang, Tyler A. Garretson, C.M. Senthil Kumar,
Robert F. Dieter, Xiao-Wen Cheng
PII:
S0168-1656(17)30678-8
DOI:
http://dx.doi.org/doi:10.1016/j.jbiotec.2017.06.397
Reference:
BIOTEC 7928
To appear in:
Journal of Biotechnology
Received date:
18-1-2017
Revised date:
14-4-2017
Accepted date:
15-6-2017
Please cite this article as: Shang, Hui, Garretson, Tyler A., Senthil Kumar, C.M., Dieter,
Robert F., Cheng, Xiao-Wen, Improved pFastBac
TM
donor plasmid vectors for higher
protein production using the Bac-to-Bac® baculovirus expression vector system.Journal
of Biotechnology http://dx.doi.org/10.1016/j.jbiotec.2017.06.397
This is a PDF file of an unedited manuscript that has been accepted for publication.
As a service to our customers we are providing this early version of the manuscript.
The manuscript will undergo copyediting, typesetting, and review of the resulting proof
before it is published in its final form. Please note that during the production process
errors may be discovered which could affect the content, and all legal disclaimers that
apply to the journal pertain.



Submitted to Journal of Biotechnology 
 
Improved pFastBac™ donor plasmid vectors for higher protein production using the Bac-
to-Bac

 baculovirus expression vector system 
 
Running title:
Improved baculovirus expression vectors 
 
Hui Shang
1,2*
, Tyler A. Garretson
2*
, C.M. Senthil Kumar
2**
, Robert F. Dieter
2
and Xiao-Wen 
Cheng
1,2 
1
Graduate Program in Cell, Molecular, and Structural Biology, 
2
Department of Microbiology, 
Miami University, Oxford, Ohio 45056 USA 
*Both authors contributed equally to this manuscript 
** Current address: Indian Institute of Spices Research, Marikunnu, P. O., Kozhikode, Kerala, 
India 673012 
Corresponding author: 
Xiao-Wen Cheng, Ph.D. 
Department of Microbiology 
32 Pearson Hall, Miami University, Oxford, Ohio 45056 USA 
Tel #: 513-529-5429 
Fax # 513-529-2431 
Email: Chengx@miamioh.edu 
Highlights 

Bac-to-Bac expresses lower protein levels than wild type AcMNPV 

The transfer vectors of Bac-to-Bac lack an 85 bp cis element and contain SV40 pA 

The polyhedrin pA is required for higher protein expression levels




Download 0,94 Mb.

Do'stlaringiz bilan baham:
  1   2   3   4   5   6   7   8   9   ...   17




Ma'lumotlar bazasi mualliflik huquqi bilan himoyalangan ©hozir.org 2024
ma'muriyatiga murojaat qiling

kiriting | ro'yxatdan o'tish
    Bosh sahifa
юртда тантана
Боғда битган
Бугун юртда
Эшитганлар жилманглар
Эшитмадим деманглар
битган бодомлар
Yangiariq tumani
qitish marakazi
Raqamli texnologiyalar
ilishida muhokamadan
tasdiqqa tavsiya
tavsiya etilgan
iqtisodiyot kafedrasi
steiermarkischen landesregierung
asarlaringizni yuboring
o'zingizning asarlaringizni
Iltimos faqat
faqat o'zingizning
steierm rkischen
landesregierung fachabteilung
rkischen landesregierung
hamshira loyihasi
loyihasi mavsum
faolyatining oqibatlari
asosiy adabiyotlar
fakulteti ahborot
ahborot havfsizligi
havfsizligi kafedrasi
fanidan bo’yicha
fakulteti iqtisodiyot
boshqaruv fakulteti
chiqarishda boshqaruv
ishlab chiqarishda
iqtisodiyot fakultet
multiservis tarmoqlari
fanidan asosiy
Uzbek fanidan
mavzulari potok
asosidagi multiservis
'aliyyil a'ziym
billahil 'aliyyil
illaa billahil
quvvata illaa
falah' deganida
Kompyuter savodxonligi
bo’yicha mustaqil
'alal falah'
Hayya 'alal
'alas soloh
Hayya 'alas
mavsum boyicha


yuklab olish