Phylogeny of the genus gossypium and genome origin

Inference of genome origin of polyploid cottons  

 

The genome origin of the Gossypium polyploid species was inferred by calculating 

repeated sequence correspondence (RSC) between a diploid and a polyploid according to 

Zhang and Dvorak (1991, 1992). 

 

(1) Character bands: To simplify the procedure of genome origin inference, a band 

detected only in one diploid genome group was defined a genome-specific band (GSB). 

If a GSB was also observed in a polyploid species, the band was defined a genome-

marker band (GMB). Similarly, if a band was detected only in a single taxon (species), 

but not in others, it was considered to be species-specific band (SSB). Further, if a SSB 

was also detected in a polyploid species, it was defined a species-marker band (SMB). 

Therefore, the GMBs or GMBs could be used as diagnostic tools for identification of the 

genome origin of the Gossypium polyploids. 

(2) RSC: The RSC is calculated using the following formula:  

                   RSC = 

∑SMBs / ∑SSBs   or    RSC = ∑GMBs / ∑GSBs       

 

 

 

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An RSC value reflects a relationship between a diploid species and the polyploid species 

under study. The value of an RSC ranges from zero to one. If none of GSBs of a diploid 

is encountered in a polyploid, the RSC is 0, suggesting that it is unlikely that the 

polyploid contains a genome from the diploid. However, if all are encountered in a 

polyploidy, RSC is 1, suggesting that the one or more of the genomes of the polyploid 

likely originated from the diploid.  

  

 

 

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