1 O‘zbekiston respublikasi oliy va o‘rta ta’lim vazirligi

 
 
 
 
 
 
 
 

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314 
Mundarija 
Bet 
Muqaddima.............................................................................................................2 
So‘z 
boshi.................................................................................................................3 
I – qism. Virusologiya predmeti va tarixi.........................................................4 
1-bob Virusologiya predmeti va viruslarga ta’rif.........................................4 
1.1. Virusologiyaning tarmoqlari.............................................................4 
1.2. Viruslar haqida ba’zi ko‘zga ko‘ringan mutaxassis olimlarning 
fikirlari....................................................................................................5 
1.3. Virusologiya soxasidagi ba’zi kashfiyotlar...................................10 
2-bob Virusologiyaning rivojlanish tarixi.................................................12 
2.1. Virusologiyaning viruslarni ochilishigacha bo‘lgan tarixi.........12 
2.2. Viruslarni ochilishi.........................................................................15 
2.3. Virusologiyaning rivojlanish bosqichlari....................................20 
2.4. Viruslar tabiati haqidagi konsepsiyaning rivojlanishi..........28 
2.5. Viruslarning ahamiyati.....................................................................30 
2.6. Viruslarning kelib chiqishi............................................................32 
2.7. Viruslarni ishlatilishi..................................................................33 
II -qism. Viruslar molekulyar biologiyasi asoslari.......................................38 
3-bob. Viruslarni ajratish va biologik tozalash.........................................38 
3.1. Viruslarni ajratib olish...............................................................39 
3.2. Fitopatogen viruslarni indikator o‘simliklarga yuqtirib identifikatsiya 
qilish..............................................................................53 
3.3. viruslarni toza preparatlarini olish...........................................59 
4-bob. Viruslarni fizik va kimyoviy usullarda tozalash..............................64 
4.1. Viruslarning tozalik mezonlari....................................................64 
4.2. Virus ajratishni optimallashtirish..............................................67 
4.3. Virus tozalash metodlarining imkoniyatlari haqida...................69 
4.4. 
Viruslarni 
ajratish 
va 
tozalashning 
fizik-kimyoviy 
metodlari...................................................................................................70 
4.5. Gelfiltratsiya..................................................................................72 
4.6.
 
Tamaki mozaikasi virusining qisman tozalangan preparatini virusning 
izo 
elektrik 
nuqtasida 
(i.e.n.) 
da 
olish............................................................................................................83 
4.7. Tamaki mozaikasi virusini tuz yordamida cho‘ktirib qisman tozalangan 
preparatini olish.................................................................85 
4.8. TMV ning differensial sentrifugalash metodi bilan toza preparatni 
olish.......................................................................................85 
4.9.TMV ning "saxaroza gradienti konsentratsiyasida sentri-fugalash" 
usulida tozalash.......................................................................86 
4.10. TMV
 
bilan kasallangan o‘simliklardan biospetsifik xromatografiya 
usulida toza virus ajratish.........................................90 

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